Biotechnology : Principles and Processes MCQs | NCERT Biology

Read the book NCERT Biology class-12 (chapter- 9): Biotechnology : Principles and Processes from here.

1. Question

Select the correct statement.

1. The tet^R gene in pBR322 can be cleaved by Pvul and Pstl.
   
2.  One can ligate a foreign DNA at BamHI site of tetracycline resistance gene in pBR322.
   
3.  When a rDNA is inserted within the coding sequence of ẞ-galactosidase, it results into insertional activation.
   
4. The rop codes for the polysaccharides involved in the replication of the plasmid.

In DNA fingerprinting, differences in some specific regions in DNA sequence are identified using a specific type of probes, which are:

Find out incorrect match:

A device which provides the optimal conditions for achieving the desired product by providing optimal growth conditions (temperature, pH, substrate, salt, vitamins, oxygen) is called –

Given below are two statements:
Statement I : Restriction endonuclease is not an important tool of biotechnology.
Statement II : Ligase enzyme is responsible for joining of DNA fragment.

In the light of the above statements, choose the correct answer from the options given below:

In gel electrophoresis, all the DNA fragments move towards the base of the gel with variable velocity because :

In a vector which of the following is helpful in selection of transformants ?

Statement-I : Biolistic method is used for gene transfer in plant.
Statement-II : DNA is coated on Gold and Tungsten Particle in Gene Gun method.

If a pBR322 vector is cut by Pst I enzyme then which of its marker gene shows insertional inactivation:

DNA or RNA fragment tagged with a radioactive molecule is called :

Given below are two statements:
Statement I: Some plasmids may have only one or two copies per cell whereas others may have 15–100 copies per cell.
Statement II: Plasmids and Bacteriophage have the ability to replicate within bacterial cells independent of the control of chromosomal DNA.

Choose the correct answer from the option given below.

Which one of the following is the incorrect match:

Select the correct statement for the given diagram:

Select the correct statement for isolation of genetic material (DNA)

The DNA molecule to which the gene of interest is integrated for cloning is called :

Thermostable DNA polymerase is taq polymerase. Taq are related with:

How many statements are correct:

1. In biotechnology ligases, host organism are a type of  key tools
2. Restriction enzymes belong to a larger class of enzymes called nucleases
3. Only some restriction endouclease functions by inspecting the length of a DNA sequence
4. Plasmid is autonomously replicating circular extra chromosomal DNA